The latest trailer for Guardians of the Galaxy Vol. 3 featured a reference to one of Thanos’ devious past acts.

While the Mad Titan is no longer at large, his impact is still being felt throughout the MCU, and particularly with the Guardians.

After all, Gamora and Nebula were both raised by Thanos, and Peter Quill is still grieving the loss of his Gamora at Thanos’ hands.

While the Guardians of the Galaxy are set to confront a new antagonist in Vol. 3, marketing for the anticipated threequel proves that the Titan may be gone but not forgotten.

Guardians of the Galaxy 3 Trailer Namedrops Thanos

In the latest Guardians of the Galaxy Vol. 3 trailer, Nebula recalls how Thanos replaced her eyes.

Marvel Studios

When Peter Quill notes how black her eyes are, Nebula explains that “they were replaced by my father as a method of torture.”

Marvel Studios

In the past, Nebula has talked about Thanos replacing pieces of her with cybernetics as punishment for losing a fight.

Audiences also saw a glimpse of his tortuous treatment of her on-screen in Avengers: Infinity War.

Marvel Studios

Guardians of the Galaxy Vol. 3 arrives in theaters on May 5.

This article is developing. Check back for updates!